產(chǎn)品編號 | bs-3717R-BF350 |
英文名稱 | Rabbit Anti-Phospho-p73(Tyr99)/BF350 Conjugated antibody |
中文名稱 | BF350標記的磷酸化P73腫瘤抑制基因抗體 |
別 名 | p73(Phospho-Tyr99); p73 (phospho Y99); p-p73 (phospho Y99);TP73(phospho Y99); p53 like transcription factor; p53 Related Protein; p73; TP 73; TP73; Tumor Protein p73; P73_HUMAN. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
產(chǎn)品類型 | 磷酸化抗體 |
研究領域 | 腫瘤 免疫學 信號轉(zhuǎn)導 細胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human, (predicted: Mouse, Rat, ) |
產(chǎn)品應用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 70kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human p73 around the phosphorylation site of Tyr99 [SP(p-Y)AQ] |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: P73 protein is a structural and functional homologue of p53, a tumor suppressor gene. In this study, The p73 protein, p19ras, by the yeast two-hybrid screening method. Alternative splicing of the proto-oncogene H-ras pre-mRNA has led to two distinct transcripts, Ras proteins are known to be small membrane-localized guanine nucleotide-binding proteins. However, unlike other Ras proteins, p19ras is localized in the nucleus and the cytosol and its interaction with P73 protein occurred exclusively in the nucleus. Oncogenic MDM2 (mouse double minutes 2) is a known repressor of p73 transcriptional activity. In this study, when p19ras was bound to MDM2, it further inhibited the association of MDM2 to the p73 protein. Therefore, this study presents a novel pathway of Ras signaling that occurs in the nucleus, involving p19ras and p73. Function: Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. [COFACTOR] Binds 1 zinc ion per subunit Subunit: Found in a complex with p53/TP53 and CABLES1. The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Interacts with HECW2. Isoform Beta interacts homotypically and with p53/TP53, whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma, isoform Alpha, and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9. Isoform Beta interacts with WWOX. Interacts (via SAM domain) with FBXO45 (via B30.2/SPRY domain). Interacts with YAP1 (phosphorylated form). Interacts with HCK (via SH3 domain); this inhibits TP73 activity and degradation. Subcellular Location: Nucleus. Cytoplasm. Note=Accumulates in the nucleus in response to DNA damage. Tissue Specificity: Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. Post-translational modifications: Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function. Higher levels of phosphorylation seen in the brain from patients with Huntington disease. Polyubiquitinated by RCHY1/PIRH2; leading to its degradation by the proteasome. Similarity: Belongs to the p53 family. Contains 1 SAM (sterile alpha motif) domain. Database links: Entrez Gene: 7161 Human Entrez Gene: 22062 Mouse Omim: 601990 Human SwissProt: O15350 Human SwissProt: Q9JJP2 Mouse Unigene: 697294 Human Unigene: 706990 Human Unigene: 78015 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. P73是p53基因家族的第一個成員p53基因是一個經(jīng)典的抑癌基因,與p53在結(jié)構和功能方面具有很高的相似性。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
| 少妇高潮毛片免费播放A片 十分钟做a小视频免费观看 | 波多野结衣在线播放 | 国产亲妺妺乱视频免费看 | 亚洲天堂无码在线观看 | 哔哩哔哩视频免费播放 | 污污污视频在线观看一区二区三区 | 精品人妻少妇一级毛片免费 | 国产精品 国产17 | 在线观看中文字幕码 | 久久人妻少妇嫩草av | 国产成人在线视频 | 蜜桃久久av一区 | 秘 亚洲国产精品成人网站 国产寡妇婬乱A毛片视频 | 在线观看免费黄色小视频 | 摸摸摸BBB毛毛毛片 国产一区二区三区在线 | 99精产国品在线观看 | 中文字幕精品一区二区精品 | 四川妇女真人毛片免费 | 成人性做爰AAA片免费 | 丰滿人妻一区二区三区 | 亚洲无码在线免费观看 | 爱咲れいら无码一区二区 | 17C丨国产丨精品入口永久地址 | 色婷婷AV一区二区三区大白胸 | 少妇做爰毛片免费看视频一区二区 | 人妻无码中文字幕免费蜜桃 | 色婷婷香蕉在线一区二区 | 人人操人人爱人人爽 | 午夜一区成人福利在线 | 国产高清热情视频在线 | 69精品久久久久久久 | 91久久夜色精品国产九色杨思敏 | 国产精品 在线播放 | 欧美成人在线视频人妻 | 一级特黄色一级视频 | 农村少妇无套内射视频 | 国产性猛交普通话对白 | 中文字幕曰本髙清无码 | 中文字幕免费在线观看 | 无码窝视频在线观看17c |