產(chǎn)品編號 | bs-0230R-Cy3 |
英文名稱 | Rabbit Anti-FGFR1/CD331/Cy3 Conjugated antibody |
中文名稱 | Cy3標記的堿性成纖維細胞生長因子受體1抗體 |
別 名 | bFGF R; BFGFR; C FGR; CD 331; CD331; CD331 antigen; CEK; FGFBR; FGFR 1; FGF Receptor 1; Fibroblast growth factor receptor 1; FLG; FLG protein; FLJ14326; FLT 2; FLT2; Fms like tyrosine kinase 2; Fms related tyrosine kinase 2; Fms related tyrosine kinase 2 Pfeiffer syndrome; H2; H3; H4; H5; HBGFR; Heparin binding growth factor receptor; Hydroxyaryl protein kinase; KAL 2; KAL2; MFR; N SAM; N sam tyrosine kinase; Protein tyrosine kinase; Tyrosylprotein kinase; Basic fibroblast growth factor receptor 1. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 腫瘤 細胞生物 免疫學 生長因子和激素 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human, Mouse, Rat, Cow, (predicted: Chicken, Dog, ) |
產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 88kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human BFGFR C-terminus |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, Tyr583, Tyr585, Tyr653, Tyr654, Tyr730 and Tyr766. Tyrosine 653 and 654 are important for catalytic activity of the activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma. Function: Receptor for basic fibroblast growth factor. Receptor for FGF23 in the presence of KL. A shorter form of the receptor could be a receptor for FGF1 (aFGF). Subunit: Monomer. Homodimer after ligand binding. Interacts predominantly with FGF1 and FGF2, but can also interact with FGF3, FGF4, FGF5, FGF6, FGF8, FGF10, FGF19, FGF21, FGF22 and FGF23 (in vitro). Ligand specificity is determined by tissue-specific expression of isoforms, and differences in the third Ig-like domain are crucial for ligand specificity. Affinity for fibroblast growth factors (FGFs) is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Likewise, KLB increases the affinity for FGF19, FGF21 and FGF23. Interacts (phosphorylated on Tyr-766) with PLCG1 (via SH2 domains). Interacts with FRS2A. Interacts (via C-terminus) with NEDD4 (via WW3 domain). Interacts with KL. Interacts with SHB (via SH2 domain) and GRB10. Interacts with KAL1; this interaction does not interfere with FGF2-binding to FGFR1, but prevents binding of heparin-bound FGF2. Interacts with SOX2 and SOX3 Subcellular Location: Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle. Note=After ligand binding, both receptor and ligand are rapidly internalized. Can translocate to the nucleus after internalization, or by translocation from the endoplasmic reticulum or Golgi apparatus to the cytosol, and from there to the nucleus. Tissue Specificity: Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. Post-translational modifications: Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor. DISEASE: Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) ; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly. Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain. Database links: Entrez Gene: 2260 Human Entrez Gene: 14182 Mouse Omim: 136350 Human SwissProt: P11362 Human SwissProt: P16092 Mouse Unigene: 264887 Human Unigene: 265716 Mouse Unigene: 207203 Rat Unigene: 9797 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 堿性成纖維細胞生長因子(bFGF)是一種多功能的生長因子,具有促進細胞有絲分裂和誘導新血管形成作用,和其受體(FGFR1)堿性成纖維細胞生長因子受體結合之后,在人體各組織中具有廣泛的生物學活性和生理病理作用.越來越多的研究表明腫瘤細胞中bFGF-R1過度表達對腫瘤發(fā)生發(fā)展具有重要意義。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
| 农村妇女一区二区 | 国产精品久久久久久久一区探花 | 午夜福利合集1000 | 午夜精品A片一二三区蜜臀 欧美一区二区三欧A片直播 | 老熟女多毛一区二区三区四区五区 | 国产真实伦子伦老人视频 | 成人人人人人欧美片做爰 | 麻豆精品秘 国产传媒视频 搡BBB搡BBB免费观看 | 国产伦码精品一区二区 | 在线区啪国自产中文字幕 | 绿色成人免费AV网站 | 国产一级a毛一级a毛观看视频网站 | 波多野结衣乳巨码在线直播 | 中国女人缸交一级A片 | 人妻饥渴偷公乱中文字幕 | 安徽扫搡BBBB揉BBBB | 成人免看一级a一片A片 | 五月天韩国亚洲三小时导航 | 国产精品一区在线观看 | AV一区二区在线观看 | 美国一区二区三区大黄片 | 午夜精品A片一区二区三区老狼 | 在线亚洲无码高清视频 | 日本一本久道熟妇人妻无码 | 强伦轩一区二区三区免费看 | 国产全黄裸体一级A片 | www.东京热.com| 成人h精品动漫一区二区三区 | 在线视频2018二页 | 拍国产真实乱人偷精品 | 免费看的黄色视频在线观看 | 干丝袜美女自慰高潮 | 久久亚麻亚洲蜜桃臀 | 成人小黄书免费网站入口3D | 国产精品高潮呻吟AV无码 | 西西4444WWW无码精品 | 免费黃色三級片在线观看 | 女人高潮天天躁夜夜躁 | 成人毛片18女人毛片免费看电影 | 毛片在线免费观看视频 |