| 產(chǎn)品編號 | bs-4730R-AP |
| 英文名稱 | Rabbit Anti-NADPH oxidase 4/AP Conjugated antibody |
| 中文名稱 | 堿性磷酸酶（AP）標(biāo)記的NADPH氧化酶4抗體 |
| 別 名 | Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX 1; KOX; Kox-1; NADPH; NADPH oxidase 4; Nox4; NOX4_HUMAN; Renal NAD(P)H-oxidase; RENOX. |
| 規(guī)格價(jià)格 | 100ul/2980元 購買 大包裝/詢價(jià) |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 心血管 細(xì)胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, |
| 產(chǎn)品應(yīng)用 | WB=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 67kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NOX4 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Nox4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases Nox4 expression, which suggests a role for Nox-4 in vascular oxidative stress response. Function: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity. Subunit: Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein (Probable). Cell junction, focal adhesion (Probable). Nucleus (Probable). Note=May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus. Tissue Specificity: Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord. Post-translational modifications: Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function. Similarity: Contains 1 FAD-binding FR-type domain. Contains 1 ferric oxidoreductase domain. Database links: Entrez Gene: 50507 Human Entrez Gene: 50490 Mouse Omim: 605261 Human SwissProt: Q9NPH5 Human SwissProt: Q9JHI8 Mouse Unigene: 371036 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 還原型輔酶煙酰胺腺嘌呤二核苷酸磷酸(NADPH)oxidase 4:還原型輔酶煙胺腺嘌呤二核苷酸（Nicotinamide adenine dinucleotide reduced, NADH）位于線粒體膜內(nèi)，是細(xì)胞能量代謝所必需的輔酶，主要功能是經(jīng)電子傳遞鏈產(chǎn)生ATP。NADH在維持細(xì)胞生長、分化和能量代謝中起重要的作用。 |
