| 產(chǎn)品編號 | bs-13106R-BF647 |
| 英文名稱 | Rabbit Anti-ERp72/BF647 Conjugated antibody |
| 中文名稱 | BF647標記的內質網(wǎng)蛋白ERp72抗體 |
| 別 名 | Calcium binding protein intestinal related; Endoplasmic reticulum resident protein 70; Endoplasmic reticulum resident protein 72; ER protein 70; ER protein 72; ERP 70; ERp 72; ERp-72; ERP70; ERp72; PDIA 4; PDIA4; PDIA4_HUMAN; PDIR; Protein disulfide isomerase A4; Protein disulfide isomerase associated 4; Protein disulfide isomerase family A member 4; Protein disulfide isomerase related protein; Protein disulfide-isomerase A4; Protein ERp 72; Protein ERp72. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 腫瘤 細胞生物 信號轉導 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Cow, Rabbit, Sheep, Monkey, ) |
| 產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 71kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ERp72 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Mammals defend themselves against intracellular pathogens through presentation of cytoplasmically derived short pathogenic peptides to the cell surface of cytotoxic T lymphocytes, which subsequently leads to cytotoxic events with respect to the affected cell. Antigen presentation is mediated by major histocompatibility complex (MHC) class I molecules, which bind and coordinate short pathogenic peptides. The proper folding and assembly of MHC class I molecules in the endoplasmic reticulum (ER) involve a number of components. MHC class I molecules assemble in the ER with chaperones before binding to the transporter associated with antigen processing (TAP) protein. ERp57 is a component of the MHC class I pathway that appears to interact with MHC class I molecules before they associate with TAP. ERp72, also designated protein disulfide-isomerase A4, is involved in the catalysis of protein -S-S- bond rearrangement. ERp57 and ERp72 may act as proteases, protein disulfide isomerases, phospholipases or a combination of these. Subunit: Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Subcellular Location: Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Similarity: Belongs to the protein disulfide isomerase family. Contains 3 thioredoxin domains. Database links: Entrez Gene: 9601 Human Entrez Gene: 12304 Mouse SwissProt: P13667 Human SwissProt: P08003 Mouse Unigene: 93659 Human Unigene: 2442 Mouse Unigene: 39305 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
