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Rabbit Anti-GRK4/BF647 Conjugated antibody (bs-16318R-BF647)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-16318R-BF647
英文名稱 Rabbit Anti-GRK4/BF647 Conjugated antibody
中文名稱 BF647標(biāo)記的G蛋白偶聯(lián)受體激酶4抗體
別    名 G protein coupled receptor kinase 4; G protein-coupled receptor kinase 2 (Drosophila)-like; G protein-coupled receptor kinase 4; G protein-coupled receptor kinase GRK4; G proteinm coupled receptor kinase GRK4; GPRK2L; GPRK4; GRK4; GRK4_HUMAN; GRK4a; ITI1.  
規(guī)格價(jià)格 100ul/2980元 購買        大包裝/詢價(jià)
說 明 書 100ul  
研究領(lǐng)域 細(xì)胞生物  信號(hào)轉(zhuǎn)導(dǎo)  激酶和磷酸酶  G蛋白偶聯(lián)受體  G蛋白信號(hào)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse,  (predicted: Human, Rat, Chicken, )
產(chǎn)品應(yīng)用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 67kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GRK4
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Function:
Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2.

Subcellular Location:
Cytoplasm. Cytoplasm; cell cortex. Both at the cell surface and dispersed in the cytoplasm under basal conditions. Receptor stimulation results in the internalization of GRK4 to the perinuclear area, where co-localization with DRD3 is observed strongly at 5 and 15 minutes. DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.

Tissue Specificity:
Isoform 1, isoform 2, isoform 3, and isoform 4 are expressed in testis. Isoform 4 is expressed in myometrium.

Post-translational modifications:
Palmitoylated.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain.

Database links:

Entrez Gene: 2868 Human

Entrez Gene: 14772 Mouse

Entrez Gene: 59077 Rat

Omim: 137026 Human

SwissProt: P32298 Human

SwissProt: O70291 Mouse

SwissProt: P70507 Rat

Unigene: 32959 Human

Unigene: 117076 Mouse

Unigene: 54517 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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