產(chǎn)品編號 | bs-19884R-HRP |
英文名稱 | Rabbit Anti-PARP10/HRP Conjugated antibody |
中文名稱 | 辣根過氧化物酶標記的多腺苷二磷酸多聚酶PARP10抗體 |
別 名 | ADP ribosyltransferase diphtheria toxin like 10; ARTD10; PARP 10; Poly (ADP ribose) polymerase family member 10; Poly [ADP ribose] polymerase 10. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 表觀遺傳學 G蛋白信號 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human, |
產(chǎn)品應用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 110kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human PARP10 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: Poly(ADP-ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP-ribose to histones. PARPs can also function as transcriptional cofactors (Yu et al., 2005 [PubMed 15674325]).[supplied by OMIM, Mar 2008] Function: May play a role in cell proliferation. May be required for the maintenance of cell cycle progression. Subcellular Location: Nucleus.Cytoplasm. Tissue Specificity: Highly expressed in spleen and thymus. Intermediate levels in liver, kidney, pancreas, prostate, testis, ovary, intestine, and leukocytes. Low expression in heart, brain, placenta, lung, skeletal muscle, and colon. Post-translational modifications: Stimulated through its phosphorylation by CDK2. Acquires CDK-dependent phosphorylation through late-G1 to S phase, and from prometaphase to cytokinesis in the nucleolar organizing regions. Phosphorylation is suppressed in growth-arrested cells. Similarity: Contains 1 PARP catalytic domain. Database links: Entrez Gene: 84875 Human Entrez Gene: 671535 Mouse Omim: 609564 Human SwissProt: Q53GL7 Human Unigene: 348609 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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