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Rabbit Anti-Phospho-MKP1 (Ser296)/AP Conjugated antibody (bs-23272R-AP)
訂購熱線:400-901-9800
訂購郵箱:sales@bioss.com.cn
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產品編號 bs-23272R-AP
英文名稱 Rabbit Anti-Phospho-MKP1 (Ser296)/AP Conjugated antibody
中文名稱 堿性磷酸酶(AP)標記的磷酸化絲裂原活化蛋白激酶磷酸酶-1抗體
別    名 DUSP1 (phospho S296); MKP1 (Phospho Ser296); MKP1 (Phospho S296); Mitogen activated protein kinase phosphatase 1; CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUSP 1; EC 3.1.3.16; EC 3.1.3.48; HVH 1; HVH1; MAP kinase phosphatase 1; MKP 1; MKP1; MKP-1; Protein tyrosine phosphatase CL100; PTPN 10; PTPN10; Serine/threonine specific protein phosphatase; VH 1; VH1; 3ch134; Mkp1; Ptpn16.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
產品類型 磷酸化抗體 
研究領域 腫瘤  免疫學  信號轉導  細胞凋亡  轉錄調節(jié)因子  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應
產品應用 WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 39kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Phospho-MKP1 (Ser296)
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Constituents: 0.05M TBS, pH 8.0 with 10mg/ml BSA and 0.05% NaN3, 50% glycerol. Or Lyophilized. Buffer = 0.05M TBS, pH 8.0 with 10mg/ml BSA and 0.05% NaN3. Reconstitute with sterile distilled water.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues.

Function:
Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185'.

Tissue Specificity:
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.

Similarity:
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 rhodanese domain.
Contains 1 tyrosine-protein phosphatase domain.

Database links:

Entrez Gene: 1843 Human

Entrez Gene: 19252 Mouse

Entrez Gene: 114856 Rat

Omim: 600714 Human

SwissProt: P28562 Human

SwissProt: P28563 Mouse

SwissProt: Q64623 Rat

Unigene: 171695 Human

Unigene: 239041 Mouse

Unigene: 98260 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

MKPs是一類絲氨酸/蘇氨酸和酪氨酸雙重底物特異性的磷酸酶 ,對于絲裂素活化蛋白激酶活性的調節(jié)起著十分重要的作用,可使絲裂素活化蛋白激酶上的蘇氨酸/酪氨酸去磷酸化失活。目前研究發(fā)現(xiàn)MKPs分別有MKP-1、MKP-2、MKP-3及MKP4-6。
MKPs受MAPK信號通路中多種成分的誘導 ,決定了它與MAPK之間作用的特異性。 通過去磷酸化作用調節(jié)MAPK信號途徑的活性 ,確保了細胞內信號的精確傳遞 ,參與了多種主要的細胞功能的調節(jié)。
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